FIGURE 7:

Nuclear exportation of ZO-2 induced by PKCε triggers TEAD translocation from the nucleus to the cytoplasm. (A) Representative images obtained from three independent experiments of the immunofluorescence of ZO-2 (a–f) and TEAD (g–l) in control sparse cultures of parental MDCK cells (first column), treated for 24 h with 2 μM nPKCε permeable activating peptide (εV1-7) that activates the nuclear ZO-2 exportation (second column), or with 2 μM nPKCε permeable inhibitor peptide (εν1-2) that blocks the nuclear exportation of ZO-2 (third column). Nuclei were stained with DAPI. (B) Representative images obtained from three independent experiments of the immunofluorescence of intestinal epithelial cells IEC-18. ZO-2 (a–f) and TEAD (g–l) in control sparse cultures of IEC-18 cells (first column) treated for 24 h with 2 μM nPKCε permeable activating peptide (εV1-7) that activates the nuclear ZO-2 exportation (second column) or with 2 μM nPKCε permeable inhibitor peptide (εν1-2) that blocks the nuclear exportation of ZO-2 (third column). Nuclei were stained with DAPI. (C) Quantification of nuclear TEAD immunofluorescence per cell in arbitrary units (AU) of the experiment described in A. At least 50 nuclei were analyzed per experimental condition. Statistical analysis done with one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05; ****p < 0.0001. (D) Quantification of nuclear ZO-2 immunofluorescence per cell in arbitrary units (AU) of the experiment described in B. At least 50 nuclei were analyzed per experimental condition. Statistical analysis done with one-way ANOVA followed by Tukey’s multiple comparisons test, **p < 0.01; *** p < 0.001; ****p < 0.0001. (E) Quantification of nuclear TEAD immunofluorescence per cell in arbitrary units (AU), of the experiment described in B. At least 50 nuclei were analyzed per experimental condition. Statistical analysis done with one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05; ***p < 0.001.