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. 2021 Jul 15;32(15):1347–1358. doi: 10.1091/mbc.E20-07-0470

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© 2021 Gallego-Gutiérrez et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 8: — Nuclear accumulation of ZO-2 due to NES inactivation has no effect on nuclear concentration of TEAD. (A) Representative immunofluorescence images derived from three independent experiments of sparse parental MDCK cells 48 h after transfection with HA-cZO-2 or a construct where the four NES of cZO-2 were mutated (HA-cZO-2 MutNES 0,1,2,3). First column, HA staining; second column, TEAD staining. Observe the strong HA staining present in the nucleus of a cell transfected with HA-cZO-2 MutNES 0,1,2,3 in comparison to that of cells transfected with HA-ZO-2. Arrows, nuclei of transfected cells. (B) Quantification of nuclear TEAD immunofluorescence in cells transfected with HA-cZO-2 or HA-cZO-2 MutNES 0,1,2,3. AU, arbitrary units. Thirty-five nuclei of transfected cells were analyzed per experimental condition. Statistical analysis done with Students t test, ns, nonsignificant. Each bar shows the mean ± SEM.