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(A) Heatmap displaying the overall amino acid frequencies in each of the P4−P4′ positions of a positive set of peptides cleaved by human TMPRSS2. Positive enrichment is observed as red, and zero was set to yellow. (B) Total spectral counts determined by LC-MS/MS highlight peptide sequences as high turnover rates substrates for TMPRSS2. (C) IceLogo depicting the extended substrate specificity of human TMPRSS2 based on 205 cleavage events detected through MSP-MS analysis.
