FIGURE 1:

SAXS analysis of HD-PTP V domain. (A) Domain organization of HD-PTP including N-terminal Bro1 domain (residues 8–361), V domain (residues 366–704), histidine/PRR domain (residues 705–1128), phosphatase domain (residues 1192–1452), and C-terminal PEST domain (residues 1509–1636). Proteins known to interact with those domains are indicated. (B) Structure cartoon of the crystal structures of the HD-PTP V domain (PDB: 5LM1 [green], 5LM2 [wheat]). (C) Solution model for the HD-PTP V domain based on chimera of crystal structures and computational modeling missing loops colored from N- to C-termini using blue–red spectrum. (D) Ab initio envelope of the HD-PTP V domain in solution from SAXS data. (E) Guinier region of SAXS data of V^HDPTP (4 mg/ml) as well as residuals to confirm little to no aggregation. At the bottom is the experimental and simulated pair distance distribution function P(r). R_g and D_max determination from P(r) curves and χ² values comparing experimental data with predicted P(r) curves of crystal structures and the solution model in C.