Orthologue clustering among races in Verticillium dahliae. (A) Phylogenetic tree of three races in V. dahliae. The phylogenetic tree was constructed using 6,238 concatenated single-copy orthologues using the maximum likelihood phylogeny with 1,000 bootstrap replicates. Verticillium alfalfae was used as an outgroup. (B) Investigation of orthologue clustering within and among race 2 in V. dahliae. Orthologues were clustered by OrthoMCL with both coverage and identities of >70%. The relationship of the orthologue among races is shown in a three-dimensional Venn diagram, with the common orthologues within race 3 (7,475 orthologues), race 2 (10,097 orthologues), and race 1 (9,535 orthologues), respectively. (C) GO annotation of the specific orthologues among three races in Verticillium. The significant clusters were selected by the Pearson chi-square test; P < 0.05.