FIG 5.
The expression and fusion activity of YLMY motif tyrosine residue mutants. (A) Western blot analysis of F YLMY mutants expressed in BSR-T7/5 cells. The cells were infected with virus at 0.1 MOI and cell lysates collected at the indicated times. The position of F0 and F1 are indicated by arrows in the left margins. (B) Conventional syncytium formation assay was induced by viral infection of Vero cells. After infection by mutant viruses, the cells were fixed with Giemsa solution. Syncytia were counted using a microscope. Each column and error bar represent the mean ± SD of syncytia for three independent experiments.