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. 2021 Jan 15;11(6):3510–3515. doi: 10.1039/d0ra10878b
PRPPa
Enzyme k cat (s−1) K m (μM) k cat/Km (μM−1 s−1)
NucV 1.34 ± 0.07 21.4 ± 3.0 0.062
ScAPRT 9.72 ± 0.47 9.8 ± 2.0 0.987
a

The constants were determined at the fixed concentration of 500 μM adenine with varied PRPP catalyzed by 1 μM enzyme.

b

The constants were determined at the fixed concentration of 500 μM PRPP with varied adenine catalyzed by 1 μM enzyme.