TABLE 1.
Alignment of translated precursor peptide amino acid sequences from different bacterial genera and strains
| Organism and straina | Darobactin type | Sequenceb |
|---|---|---|
| * * * * * * * * * * * * * * * * * * * *** ** **** **** * * | ||
| P. khanii DSM3369 | A | MHNTLNETVKTQEALNSLAASFKETELSITDKALNELSNKPKIPEITA WNWSKSF QEI |
| P. heterorhabditis VMG (1) | B | MQNIPIETCKDQELLNSLVTSFKGTELSITEKALDELANNTEIPEINA WNWTKRF PI |
| P. heterorhabditis VMG (2) | A | MQNILVETCKTQEALNSLAASFKETELSITEKALNELSSKPKIPEITA WNWSKSF QEI |
| P. thracensis DSM15199 (1) | (F) | MQKIPTETCKNQELLNYLVTSFKGTELSITKKRLDELVNKTDIPDMTT WKWSKNL PI |
| P. thracensis DSM15199 (2) | A | MHNTSNEIIKTQEALNSLAASFKETELSITDKALNELSNKPNIPEITA WNWSKSF QEI |
| ************* **** * ** * ** ************* | ||
| Vibrio vulnificus | A | MIIVEKEKVSISEKLDALMSSFSEMNLELRKFDQEKVNSINIAPPITA WNWSKSF |
| Pseudoalteromonas luteoviolacea | A | MIVEAPKEKVSISEKLDALKSSFSNQTLNIANVDQARVDSISVAPPITA WNWSKSF EK |
| * * * ** * ** * * **** ** ** | ||
| Y. enterocolitica | A | MFTSNQSNERINNTHLMALKTKLESLEQSFKNNLFSINDHEIENLRRSNSNNQITA WNWSKSF TQQ |
| Y. enterocolitica | D | MYTSHQSDLNTNNGKLIALKTKLEALDESFENNSLHISYDEIEKIKNNSLKSKITA WNWSRSF AEE |
| Y. bercovieri | A | MYTSHHTDRKTSNSNLMALKAKLESLDQSFKSNLLSISDHEIENLKNNNFNNEITA WNWSKSF TQQ |
| Y. bercovieri | E | MYTSHHTDRKTSNSNLMALKAKLESLDQSFKSNLLSISDHEIENLKNNNFNNEITAWSWSKSFTQQ |
| Y. frederiksenii | A | MYTSHQPEKKTSNTNLIALRTKLESLEESFKNSGLSIDAQEIENLKNSESENKITA WNWSKSF TQQ |
| Y. rohdei | A | MYTSRNPDGEIIPSNIMALLTKLGSLDESFKNNALTINNNEIENLKNSEVNNKITA WNWSKSF TQQ |
| Y. aldovae | A | MYNSTSHQKSHSVNNATALRSKLLSLQESFKSIPIHININKIEDLINSKSNNKITA WNWSKSF SQD |
| Y. aldovae | D | MFTSNQSNERINNAHLMALKAKLESLDESFKNNTLHISDNEIEKIKSNTLRSKITA WNWSRSF AEE |
| Y. massiliensis | A | MSISFQHQRKNNDQNLLALKSKLQSLGESFSHHSLYISNSELDKIRNSLAKTKITA WNWSKSF TEN |
| Y. pseudotuberculosis | D | MNPSSQSTVEKNNVNLIKLKSKLQSLEESFKNNPLYITSNEIDEVKNNTLHTKITA WNWSRSF AED |
| Y. pseudotuberculosis | C | MNPSSQSVVEKSNVNLIKLKSKLKSLEESFKNNPLYITSNEIDEIKNNTLHSKITA WSWSRSF AED |
a
P., Photorhabdus; Y., Yersinia. Alignments were done seperately for Photorhabdus, Vibrio and Pseudoalteromonas, and Yersinia strains.
b
The precursor sequence is given, and the core amino acid sequence of the heptapeptide is underlined. The DAR F precursor peptide sequence (gray shading) is displayed in the alignment; however, due to strong sequence aberration, it was not taken into account for the amino acid identity, which is indicated by the asterisks.