TABLE 3.

MICs of darobactin A and B against pathogens, a gut commensal, darobactin-resistant strains, and human cell lines

Organism and strain or cell line	MIC (μg/ml) of:
	DAR A	DAR B
Pathogens
Klebsiella pneumoniae ATCC 700603	2	2
Salmonella enterica serovar Typhimurium LT2 ATCC 19585a	2	2
Escherichia coli ATCC 25922	2	2
E. coli MG1655	4	8
E. coli AR350 (mcr-1)	2	4
Pseudomonas aeruginosa PAO1	2	2
P. aeruginosa PA14	16	8
Acinetobacter baumannii ATCC 17978	8	4
A. baumannii LAC-4	8	4
A. baumannii AB5075-UW	32	8
A. baumannii AB307-0294	32	16
A. baumannii ACICU	64	32
A. baumannii AB057	64	32
A. baumannii AYE	128	64
Moraxella catarrhalis ATCC 25238	8	8
Enterobacter cloacae ATCC 13047	32	128
Proteus mirabilis ATCC 7002	64	128
Stenotrophomonas maltophilia ATCC 13637	>128	>128
Staphylococcus aureus HG003	>128	>128
Gram-negative gut commensal
Bacteroides fragilis ATCC 25285a	>128	>128
Darobactin-resistant E. coli strains (bamA mutants)
E. coli MG1655 bamA mutant strain 1	>128	>128
E. coli MG1655 bamA mutant strain 2	>128	>128
E. coli MG1655 bamA mutant strain 3	>128	>128
Human cell lines
FaDu	>128	>128
Hek293	>128	>128
HepG2	>128	>128

^aGrown under anaerobic conditions.