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. 2021 Dec 22;9(3):e01497-21. doi: 10.1128/spectrum.01497-21

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Copyright © 2021 Davidsson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Expression of CCL17, CCL18, and PD-L1 in unstimulated macrophages (control) and macrophages stimulated with C. acnes type IA or type II. (A) mRNA expression in unstimulated control and C. acnes type IA or type II stimulated macrophages, presented as mean +/- SD of log ΔCT (i.e., the mRNA expression of the gene of interest minus the expression of the housekeeping gene POLR2F). The lower the ΔCt value, the higher the expression of the mRNA of interest. (B) Protein expression in unstimulated control and C. acnes type IA or type II stimulated macrophages, presented as mean +/- SD of log pg/ml. The statistical analysis were performed using repeated measurements ANOVA with two within subject factors (treatment and replicates). The treatments were unstimulated (control), stimulated with C. acnes type IA or with C. acnes type II. The number of replicates were three, done with three different macrophage donors. *P < 0.05, **P < 0.01.