TABLE 1.
qPCR determination of specific cell types (swine-specific genes) and receptors in ALI-PRECs and pig tracheaa
| Gene | NCBI accession no. | Specificity | ALI-PRECs | Trachea |
|---|---|---|---|---|
| PCNA | NM_001291925.1 | Proliferating cells | + (2/2) | + (2/2) |
| MUCIN2 | XM_021082584.1 | Goblet cells | + (2/2) | + (2/2) |
| CHGA | NM_001164005.2 | Neuroendocrine cells | + (2/2) | + (2/2) |
| LGR5 | XM_021090898.1 | Stem cells | + (2/2) | + (2/2) |
| SPIB isoform 1 | XM_003127365.3 | Brush cells | + (2/2) | + (2/2) |
| GP2 | XM_003124571.4 | Microfold cells | + (2/2) | + (2/2) |
| KRT5 | XM_003126173.4 | Basal cells | + (2/2) | + (2/2) |
| DPP4/CD26 | NM_214257.1 | Coronavirus receptors | + (2/2) | + (2/2) |
| APN/ANPEP | NM_214277.1 | Coronavirus receptors | + (2/2) | + (2/2) |
| ACE2 | NM_001123070.1 | Coronavirus receptors | + (2/2) | + (2/2) |
| CEACAM1/CD66a | XM_021094420.1 | Coronavirus receptors | + (2/2) | + (2/2) |
a
The presence and absence of the receptors are represented by + and −, respectively. Values in parentheses were the number of biological samples tested positive per total tested.