FIG 2.
The role of neutrophils in the Mp-mediated lung injury and inflammation. The mice were treated intraperitoneally with an anti-Ly6G antibody (Ab) (100 μg/mouse) or isotype control Ab and challenged with Mp (6 × 107 CFU) on 24 h after Ab treatment. Nontreat group indicated mice treated with PBS intranasally and without Ab treatment. (a) The DNA copies of Mp in the BALF were measured by real-time PCR 24 h after the Mp challenge. (b) At 24 h and 72 h after the Mp challenge, the level of LDH in BALF was measured. (c, d) At 24 h after the Mp challenge, (c) the levels of IL-1α, IL-6, and IL-12 p40 and, (d) the level of MMP-9 in BALF were measured by ELISA. (a–d) Each experiment was performed more than twice. Data are shown as means ± SD. (a) n = 9–10; (b–d) n = 4–5. (a–d) *, P < 0.05; **, P < 0.01 as indicated by Tukey’s test. See also Fig. S5.