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Absence of CTF18 leads to a condensation defect. (A) Nocodazole-arrested CTF18 (YPH499) and ctf18Δ (YE105) cells were assayed by fluorescence in situ hybridization using an rDNA probe. rDNA is detected by immunofluorescence (green); chromosomal DNA is stained with propidium iodide (red). A nucleus with decondensed rDNA is indicated by the arrow. (B) A total of 100 nuclei were scored for rDNA condensation status in four categories. Data were collected twice (averages are shown).
