FIG. 4.

Ctf18p throughout the cell cycle. (A) α-factor arrest-release time course for CTF18-9myc cells (YJH40.4). CTF18-9myc was detected using anti-myc antibody and compared with β-tubulin (Tub 2p) on the same blot. (B) (Left) Fluctuation in Ctf18p-9myc accumulation was determined in normalized units by comparison of band intensity relative to β-tubulin. (Right) Separate aliquots from the same time course were prepared for flow cytometry and analyzed for DNA content (data not shown) as well as being scored for nuclear morphologies. These analyses indicated the execution of S phase by the majority of cells between 30 and 60 min and the initiation of M phase between 70 and 80 min.