FIG. 8.

Candidate CTF18 orthologues and conserved RF-C boxes. (A and B) Modified output of National Center for Biotechnology Information pairwise BLAST (BLOSUM62) of representative sequences to illustrate the distribution of similarity. Roman numerals indicate RF-C homology boxes, the hatched box represents the ligase homology domain, and black bars mark the relative positions of RF-C boxes. (A) ScCtf18p versus ScRfc1p (e-value = 1e⁻¹⁵). (B) ScCtf18p versus HsCtf18p (e-value = 3e⁻³⁵). (C) Proteins from C. elegans, D. melanogaster, S. pombe, and H. sapiens were identified among the top hits from a PSI-BLAST search using S. cerevisiae Ctf18p as the query. Clustal X v1.8 (44) was used to make a multiple alignment of proteins from C. elegans, D. melanogaster, S. pombe, and H. sapiens. Using the multiple alignment, a bootstrapped neighbor-joining tree was produced. The analysis shows that Ctf18p homologs cluster away from Rfc1p homologs.