TABLE 2.
Agreement between conventional culture and 16S rRNA gene amplicon sequencing
| Sample group and UTI diagnosis according to 16S rRNA gene amplicon sequencinga | No. with indicated UTI diagnosis according to conventional cultureb |
||
|---|---|---|---|
| Likely | Possible | Unlikely | |
| All | |||
| Likely | 70 | 15 | 0 |
| Possible | 2 | 2 | 3 |
| Unlikely | 2 | 4 | 20 |
| Catheter collected | |||
| Likely | 46 | 2 | 0 |
| Possible | 1 | 1 | 1 |
| Unlikely | 1 | 1 | 3 |
| Clean catch collected | |||
| Likely | 24 | 13 | 0 |
| Possible | 1 | 1 | 2 |
| Unlikely | 1 | 3 | 17 |
Diagnosis of UTI according to 16S rRNA gene sequencing was categorized as follows: likely UTI was the presence of a uropathogen at ≥90% abundance; possible UTI was the presence of a uropathogen at between 50% and 90%; and unlikely UTI was any sample categorized as neither likely UTI nor possible UTI. UTI, urinary tract infection.
Diagnosis of UTI according to conventional culture was categorized as follows: likely UTI was growth of a single uropathogen at a count of ≥100,000 CFU/ml without growth of other organisms; possible UTI was growth of a single uropathogen at 10,000 to 99,000 CFU/ml with no other growth, growth of 2 uropathogens, or growth of at least one uropathogen along with nonuropathogen(s); and unlikely UTI was no growth or growth of nonuropathogen(s) in the absence of a uropathogen.