Fig. 6. (a) Fluorescence imaging and (b) quantification of 293T (human embryonic kidney cells) cells without and with pre-treatment of a known fucosidase inhibitor (1-deoxyfuconojirimycin) after incubation with TD-Fuc6 (10 μM) for 40 min. The excitation and emission channels used for TD-Gal6 are 488 nm and 570–630 nm, respectively. S. D. means standard deviation (n = 3). **P < 0.01, ***P < 0.001. Statistical analysis was performed using Student's unpaired t-test. All experiments were repeated three times with representative data shown.
