AMVI calculation of the proposed method.

Parameters	Developed HPLC method
Solvent consumption method (mL)	114.75
Flow rate	1.7
Run time	7.5
No. of injection for one full analysis	9
No. of potential analytes	1
Solvent consumption sample preparation (mL)	42.50
Standard prep. volume (mL)	10
No. of standard preps.	2
Sample prep. volume (mL)	2.5
No. of sample preps.	9
Total method solvent consumption	157.25
Analytical method volume intensity	157.25/1 = 157.25
% Consumption method	72.97%
% Consumption preparation	27.03%