A) Composite UMAP showing the overlay of spike-specific B cells from individuals who recovered from non-severe COVID-19 at 2–3 (left), 4–5 (middle), and 20–23 (left) weeks post-symptom onset. B) Overlay of major B cell subsets and activated/resting switched memory B cells onto the composite UMAP. C) Expression of CD80, Ki-67, and CD95 by spike-specific (S+) IgG+ B cells at the three time points. D) Distribution of the four T-bet+ subsets (as defined in Fig 5) among spike-specific IgG+ B cells at the three time points post-symptom onset. E) Expression of T-bet, FcRL5, CD11c, and CD21 by spike-specific IgG+ B cells at the three time points post-symptom onset. In all graphs, results are shown for samples collected 2–3 (n = 9), 4–5 (n = 7), and 20–23 (n = 7) weeks post-symptom onset. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.