TABLE 2.
Flow cytometric analysis of leukocytes
| Tissue | Marker | Mean % of leukocytesa ± SD in:
|
P | |
|---|---|---|---|---|
| Wild-type mice | fractalkine−/− mice | |||
| Blood | F4/80 | 18.2 ± 2.1 | 11.5 ± 0.8 | 0.01b |
| Mac-1 | 37.5 ± 2.1 | 38.8 ± 0.6 | 0.37 | |
| CD11c | 3.4 ± 0.28 | 3.5 ± 1.1 | 0.15 | |
| Pan NK | 10.2 ± 2.3 | 7.4 ± 2.8 | 0.91 | |
| B220 | 40.0 ± 0.2 | 44.0 ± 7.7 | 0.53 | |
| CD3 | 39.0 ± 3.9 | 30.7 ± 6.0 | 0.19 | |
| Lymph nodes | F4/80 | 0.3 ± 0.11 | 0.5 ± 0.06 | 0.14 |
| Mac-1 | 0.6 ± 0.2 | 0.6 ± 0.08 | 0.87 | |
| CD11c | 1.6 ± 0.21 | 1.6 ± 0.12 | 0.60 | |
| Pan NK | 1.5 ± 0.07 | 1.4 ± 0.12 | 0.87 | |
| B220 | 19.4 ± 5.7 | 20.4 ± 3.4 | 0.80 | |
| CD3 | 79.6 ± 5.3 | 78.7 ± 3.3 | 0.83 | |
| Spleen | F4/80 | 6.6 ± 0.2 | 7.5 ± 0.9 | 0.28 |
| Mac-1 | 3.5 ± 0.01 | 5.0 ± 1.3 | 0.21 | |
| CD11c | 5.9 ± .92 | 6.5 ± 0.61 | 0.40 | |
| Pan NK | 6.4 ± 1.6 | 6.7 ± 0.91 | 0.79 | |
| B220 | 56.4 ± 4.6 | 59.3 ± 0.7 | 0.34 | |
| CD3 | 36.2 ± 5.6 | 31.6 ± 1.9 | 0.26 | |
a
The values refer to the percentage of CD45+ leukocytes in the tissue having the indicated cell surface marker.
b
The significant difference in blood F4/80 cells between wild-type and fractalkine−/− mice was seen in each of three separate experiments with three mice per group.