FIGURE 5.

METTL1 mediated m⁷G transfer RNA (tRNA) modification regulates mRNA translation through codon recognition in bladder cancer (BC). (#) Polysome profiling of the METTL1 KO, empty vector control T24 cells, METTL1 expression after knocking out METTL1 T24 cells and METTL1 KO control T24 cells. (B) Distribution of ribosome protected fragment (RPF). (C) Ribosome occupancy changes at A and A+1 sites for codons decoded by m⁷G tRNAs and non m⁷G tRNAs (****P < 0.0001). (D) Ribosome occupancy at individual codon at A and A+1 sites in METTL1 knockout and control cells. The codons were separated into three groups based on the modification of their tRNAs: codons decoded by m⁷G tRNAs (red); codons decoded by non‐m⁷G tRNAs by wobble effect which were not detected levels of their corresponding tRNAs (purple); and codons decoded by non‐m⁷G tRNAs (black). (E) Gene ontology (GO) analysis of function enrichment in biological process using the translation efficiency (TE) downregulated genes upon METTL1 KO. (F) GO analysis of function enrichment in the biological process using the TE upregulated genes upon overexpression METTL1 after METTL1 KO. (G) Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis of the TE‐downregulated genes upon METTL1 KO. (H) KEGG pathway enrichment analysis of TE‐upregulated genes upon overexpression METTL1 after METTL1 KO