TABLE 1.
Antibodies used in this study.
Antibody | Antigen | Host | Source | Application | Dilution |
---|---|---|---|---|---|
2524 | Mouse p53 | Mouse | Cell Signaling Technology | Western blot | 1:1,000 |
ChIP | 5 μg antibody/25 μg DNA | ||||
ICC | 1:2,000 | ||||
SC-365062 | GAPDH | Mouse | Santa Cruz | Western blot | 1:200 |
P53-CM5P-L | P53 | Rabbit | Leica | IHC | 1:200 |
13-0300 | GFAP | Rat | Invitrogen | ICC | 1:250 |
E0431 | Rabbit Immunoglobulin | Swine | Dako | IHC – Secondary/Biotin | 1:750 |
926-32212 | Mouse IgG | Donkey | LI-COR | Western blot – Secondary/IRDye 800CW | 1:50,000 |
A11077 | Rat IgG | Goat | Invitrogen | ICC – Secondary/Fluor 568 | 1:1,000 |