Fig. 3. Agonist activation of rat primary neuron delta receptors resembles that of GluD2 with Cbln1 and NRX1 in HEK-293T cell clusters.

(A) Representative currents recorded from cerebellar neurons with established connections upon application of 10 mM glutamate, d-serine, or glycine. (B) Comparison of glycine-activated currents in cerebellar neurons with connections, cortical neurons with connections, and cerebellar neurons without connections. (C) Representative responses of cerebellar neurons with connections to different concentrations of glycine. (D) Normalized glycine concentration-response curve for cerebellar neurons with connections (EC₅₀ = 1.48 ± 0.08 mM); the number of measurements for each concentration is shown in table S1. (E) Representative currents showing inhibition of 10 mM glycine-mediated currents by 500 μM DCKA.