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. 2021 Dec 22;7(52):eabk2200. doi: 10.1126/sciadv.abk2200

Fig. 4. Cbln1 and NRX1 induce tighter packing of the N-terminal domains of GluD2.

Fig. 4.

(A) Structure of GluD2 (modeled on GluD1; PDB: 6KSS) showing SNAP tag incorporation at position 302 (red circles) and distance between dimers. Side view (top) and close-up top-down view of 302 N-terminal domain residues (bottom). (B) Fluorescent images of HEK-293T cells transfected with GluD2 alone or GluD2, NRX1, and Cbln1 showing the intensity of donor and FRET fluorescence. (C and D) Representative fluorescence lifetimes of donor- and acceptor-labeled cells expressing GluD2 alone or GluD2, Cbln1, and NRX1; solid lines show two exponential fits. (D) Donor lifetimes from at least three different days. Data represent mean lifetimes with SEs of the mean, *P = 0.00052.