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. Author manuscript; available in PMC: 2021 Dec 22.
Published in final edited form as: Oncogene. 2021 Mar 8;40(14):2553–2566. doi: 10.1038/s41388-020-01599-z

Fig. 1. RGS12 is downregulated in both human and mouse osteosarcoma tissues.

Fig. 1.

a Real-time PCR quantification of Rgs12 mRNA levels in the bones of OSX-Cre;P53f/f/Rb1f/f and P53f/f/Rb1f/f mice. b A representative image of western blot. Whole bone lysates from OSX-Cre;P53f/f/Rb1f/f and P53f/f/Rb1f/f mice were immunoblotted with antibodies against Rgs12 and GAPDH, respectively. N=3. The Rgs12 expression is quantified by ImageJ software in the corresponding column at right. c A representative image of immunohistochemical staining of Rgs12 on mouse osteosarcoma and normal bones. Scale bar, 75 μm. N=5. The Rgs12 expression is quantified by ImageJ software in the corresponding column at right. d A representative image of immunohistochemical staining of RGS12 on different grades of human osteosarcoma (tumor grades 1 to 4) and normal bones. Scale bar, 100 μm. OS, osteosarcoma. e, Quantification of RGS12 expression by ImageJ software based on IHC staining in human osteosarcoma (tumor grades 1 to 4; Grade 1, N=27; Grade 2, N=21; Grade 3, N=6; Grade 4, N=12) and normal bones (N=32). Error bars were the means ± standard error of the mean (SEM) of triplicates from a representative experiment. *P < 0.05, **P < 0.01 and ***P < 0.001.