Figure 9. Confocal microscopy images of CC-2343 and CC-1009 showing ROS in cells growing at steady state (Panels A and B) and following exposure to approximately 31 hr of hyperoxia (Panels C and D) of CC-2343 and CC-1009.

H₂DCFDA, a nonfluorescent probe that is converted into fluorescent dichlorofluorescein (DCF) by ROS, was used to detect the ROS. The ROS is indicated by the green fluorescence, while the auto-fluorescence of the chloroplasts is displayed in red. ROS, reactive oxygen species; Chl, chlorophyll; BF, bright field. Scale bars = 10 μm.