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. 2021 Dec 18;14:7035–7051. doi: 10.2147/JIR.S335915

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© 2021 Gong et al.

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OLFM4 expression in lung epithelial cells was induced by neutrophil media. (A) MLE-12 cells were incubated with LPS (5 µg/mL) for 6, 12, 24, and 48 h, and the expression level of OLFM4 was detected by Western blot. (B) MLE-12 cells were incubated with different doses of LPS (500 ng/mL, 1 µg/mL, 5 µg/mL, 10 µg/mL) for 24 h, and OLFM4 expression was measured by Western blot. (C) BEAS-2B cells were incubated with LPS (5 µg/mL) for 6, 12, 24, or 48 h, and OLFM4 expression was measured by Western blot. (D) Murine BMDNs were purified and the purity was assessed by flow cytometry. (E) BMDNs were stimulated with or without LPS for 4h, and BMDN media was collected. MLE-12 cells were treated with BMDN media or DMEM and OLFM4 expression was measured by Western blot.