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Nucleotide sequence alignment of the region amplified in the PCR-RFLP assay. The regions from which the consensus PCR primers (MCV1 and MCV2) were chosen are underlined. The unique NcoI restriction enzyme site that is present in all PCV-2 isolates is indicated by asterisks. The sequence of PCV-2 isolate 26606 from this study is shown on top, and only differences from that sequence are indicated for the other isolates. The sequences used in the alignment are cited in the text.
