FIG. 5.

Detection and differentiation of PCV infections by a PCR-RFLP assay. (A) Results of PCR amplification of a 243-bp fragment from tissue samples that contained PCV isolates (both PCV-1 and PCV-2) but not from a negative control liver tissue sample (lane 1). (B) Results of RFLP analysis of the PCR products. Lane L, 50-bp DNA ladder; lane 1, a sample of liver tissue from a control specific-pathogen-free pig; lanes 2 to 11, tissue samples from 10 pigs with PMWS, respectively; lane 12, PK15 cells containing PCV-1; lane 13, a sample containing both PCV-1 and PCV-2. The expected PCR fragment (A) and three RFLP fragments of 243, 168, and 75 bp, respectively (B), are indicated with arrows.