TABLE 2.
Oligonucleotide primers used in the study
| Primer | Primer sequence | Application | Positiona |
|---|---|---|---|
| CV1 | 5′-AGGGCTGTGGCCTTTGTTAC-3′ | PCR, Seqb | 1336–1355 |
| CV2 | 5′-TCTTCCAATCACGCTTCTGC-3′ | PCR, Seq | 536–556 |
| CV3 | 5′-TGGTGACCGTTGCAGAGCAG-3′ | PCR, Seq | 452–471 |
| CV4 | 5′-TGGGCGGTGGACATGATGAG-3′ | PCR, Seq | 1525–1544 |
| CV1-1 | 5′-GAGGATCTGGCCAAGATGGCTG-3′ | Seq | 1674–1695 |
| CV1-2 | 5′-AGGACGAACACCTCACCTCCAG-3′ | Seq | 213–234 |
| CV2-1 | 5′-GCAGCGGGCACCCAAATACCAC-3′ | Seq | 279–300 |
| CV2-2 | 5′-ACGTATCCAAGGAGGCGTTACC-3′ | Seq | 1718–1739 |
| CV3-1 | 5′-AGACTAAAGGTGGAACTGTACC-3′ | Seq | 770–791 |
| CV3-2 | 5′-TTGTACATACATGGTTACACGG-3′ | Seq | 1083–1104 |
| CV4-1 | 5′-TGTGGACCACGTAGGCCTCGGC-3′ | Seq | 1146–1167 |
| CV4-2 | 5′-TGGTAATCAGAATACTGCGGGC-3′ | Seq | 799–820 |
| MCV1 | 5′-GCTGAACTTTTGAAAGTGAGCGGG-3′ | PCR-RFLP | 508–531 |
| MCV2 | 5′-TCACACAGTCTCAGTAGATCATCCCA-3′ | PCR-RFLP | 724–749 |
a
The relative positions of these oligonucleotide primers are indicated in Fig. 1.
b
Seq, primers used for DNA sequencing to determine the complete genome of PCV-2.