Figure 1.
T cell receptor sequences from sorted human lymph node TFH cells following mRNA vaccination
(A) Study timeline. Day 0 blood samples were obtained prior to the first dose of the vaccine and day 21 samples were taken prior to the second dose of the vaccine.
(B) Sorting strategy for TFH cells from LN aspiration samples obtained on day 60.
(C) Similarity network of the 500 most abundant TCRα sequences (left) and TCRβ sequences (right) from the lymph node TCR repertoire obtained from sorted TFH cells of 4 individual donors (01a, 04, 20, and 22) 60 days after mRNA vaccination. Each vertex corresponds to an individual TCR clonotype, which are connected to adjacent data points if they have identical VJ-segments and less than 2 mismatches in the CDR3 amino acid sequence. The size of the vertex corresponds to the vertex degree (number of neighbors).