Figure 4.

S_167–180 response in the draining lymph node following BNT162b2 vaccination

(A) Representative flow cytometry plots of subject 20 demonstrating the frequency of S_167–180 tetramer⁺ cells expressed as a percentage of total CD4⁺ T cells in the lymph node FNA sample (top row). The bottom row demonstrates CXCR5 and PD1 surface expression on the gated S_167–180 tetramer⁺ cells from the row above.

(B) The percentage of total CD4⁺ T cells that are S_167–180 tetramer⁺ in blood (red lines) and FNA (blue lines) in matched samples taken at the same time points from subjects with available sample.

(C) The percentage of CXCR5⁺PD1⁺ T cells that are S_167–180 tetramer⁺ over time in both the blood (red lines) and FNA (blue lines).