Fig. 3.

Confirmation of DEGs by qRT-PCR, western blotting, and ELISA. (A) Fold change values in RNA-seq data of selected GPM-regulated genes were compared with those in qRT-PCR data. (B) Expression of NGFR, ADM2 and α-tubulin in HUVECs exposed to GPM or DMSO for 24 h. (C) The expressions of NGFR and ADM2 in Fig. 3B were quantified by being normalized to the corresponding level of α-tubulin. *P < 0.02 versus DMSO. (D) The amount of IL-11 in the medium of HUVECs exposed to GPM or DMSO for 24 h was determined by ELISA. *P < 0.02 versus DMSO.