Fig. 3.

Effect of LPS pre-treatment on the efficiency of mRNA expression. A representative sample set of dissected spleens and livers (a) were analyzed 5 min after the administration of D-luciferin. The bioluminescent signal was quantified as total flux (p/s) in liver (b) and spleen (c), and compared between naïve and LPS-treated mice receiving modmRNA-LNP (0.32 mg-mRNA kg⁻¹) as IV injections four hours post-IV-LPS (2 mg kg⁻¹) treatment. Group size is 4 animals and error bars indicate SEM. Statistical analysis was performed by Student's t-test, comparing LPS/modmRNA-LNP to naïve/modmRNA-LNP (**P < 0.01).