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. 2021 Dec 21;16:101289. doi: 10.1016/j.tranon.2021.101289

Figure 1.

Figure 1

Kinesin superfamily protein 4A (KIF4A) is highly expressed in osteosarcoma (OS).

(a) Volcano plot of mRNA differential expression in the control vs. G0-sorted OS cells (left) and in the control vs. doxorubicin (DOX)-treated OS cells (right). The blue and red dots lying in the top sectors represent significantly down- and up-regulated mRNAs, respectively (p<0.05, log FC ≤-2 or ≥2). (b) Venn diagram of upregulated genes between control vs. G0 sorted cells and control vs. Dox treated cells. (c) For 360 up-regulated genes, protein-protein interaction (PPI) network analysis was constructed by using the STRING database and Cytoscape. (d, e) KIF4A was significantly overexpressed in OS cancer samples compared to its expression in normal bone cell samples. These date included 13 normal samples and 13 OS samples in GSE99671; 4 normal samples and 4 OS samples in GSE126209; 2 normal samples and 262 OS samples in TCGA; (*p<0.05). Red and grey colors represent tumor and normal tissues, respectively. (f) The expression of KIF4A was related with poor prognosis (overall survival [OS] (p=0.014, groups for KIF4A high expression n=214; groups for KIF4A low expression n=235), progression-free interval [PFI] (p=0.0063, groups for KIF4A high expression n=172; groups for KIF4A low expression n=200), disease-free interval [DFI] (p=0.0031, groups for KIF4A high expression n=108; groups for KIF4A low expression n=142), and disease-specific survival [DSS] (p=0.027, groups for KIF4A high expression n=211; groups for KIF4A low expression n=230)) as demonstrated by analyzing OS clinical samples using the Sangerbox software. Red and blue colors represent high expression groups and low expression groups, respectively. Data were obtained from TCGA database.