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. 2021 Dec 21;16:101289. doi: 10.1016/j.tranon.2021.101289

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© 2021 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Chondrocyte-derived exosomal miR-195 is internalized by OS cells.

(a) The expression of miR195 in chondrocytes after transfection detected using qPCR. The expression of miR-195 was significantly high in chondrocytes (**p<0.01). (b) The level of KIF4A in chondrocytes after transfection detected using western blotting. (c) The morphology of exosomes showed a bowl-shaped bilayer membrane structure using transmission electron microscopy.(d) Size distribution of exosomes measured using nanoparticle tracking analysis. (e) Exosome marker proteins CD63, CD81, and Calnexin detected using western blotting. (f) The expression of miR195 in exosomes detected using qPCR. (g) Confocal microscopy images showing that exosomal miR-195 is internalized by MG63cells (PKH26 marked exsomes in red and DAPI marked nucleus in blue, magnification 600 × ). (h) The level of miR-195 detected in U2OS and MG63 cells using qPCR. (i, j) The mRNA and protein levels of miR-195 detected in U2OS and MG63 cells using qPCR and western blotting. *p<0.05 and **p<0.01.