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. 2021 Sep 10;102(1):80–89. doi: 10.1038/s41374-021-00671-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blots of αTAT1 and Acetylated α-tubulin (Ac-Tubulin) in control (scramble siRNA transfected; siNeg) and αTAT1 knocked down cells (siATAT1). B (a) Wound healing assay to evaluate the migration ability of AM-1 cells in the control (siNeg) and siATAT1 transfected conditions. The front line of migrative AM-1 cells is represented by a yellow dotted line in each panel. The culture is stopped after 22 h. Scale bars: 50 μm. b Each dot in the graph indicates the closing ratio of the wounded area in siNeg or siATAT1 transfected condition. Statistical significance was set as **p < 0.01 (n = 6). C Dual immunocytostaining of αTAT1 (green: a, d) and Ac-Tubulin (red: b, e) in the control (siNeg) (a–c) and siATAT1 transfected (d–e) cells. Merged images with DAPI staining are shown in (c, f). Scale bars: 50 μm. D Dual immunocytostaining of Ac-Tubulin (green: a, d) and F-actin (red: b, e) in the control (siNeg) (a–c) and siATAT1 transfected (d–f) cells. Merged images with DAPI staining are shown (c, f). Allow heads show lamellipodium formation at the leading edge of the migrating cells. Scale bars: 50 μm.