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. 2021 Sep 10;102(1):80–89. doi: 10.1038/s41374-021-00671-w

Fig. 4. Analyses of the effect of TGF-β stimulation and the mechanisms of related molecules interaction on the tubulin acetylation in AM-1 cells.

Fig. 4

A Immunocytostaining of Ac-Tubulin (green) and F-actin (red) in the control and TGF-β stimulated AM-1 cells. Nuclei are stained with DAPI (blue). Ac-Tubulin expression is upregulated by the TGF-β stimulation. Scale bars: 50 μm. B (a) In situ proximity ligation assay to confirm the interaction of αTAT1 and TAK1. Colocalization of αTAT1 and TAK1 which are secondary labeled by PLA probes (red dots) in the control and TGF-β stimulated conditions. Nuclei are stained with DAPI (blue). Scale bars: 50 μm. b Bars indicate relative PLA intensity in the control and TGF-β stimulated conditions. Statistical significance was set as **p < 0.01 (n = 9).