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Induction of β3-integrin by Raf and MEK1. NIH 3T3 cells expressing ΔA-Raf:ER*, ΔRaf-1:ER*, ΔB-Raf:ER*, or ΔMEK1:ER* were either left untreated or treated with 100 nM 4-HT for 48 h, at which time the cells were harvested and stained for the cell surface expression of β3-integrin as described in Materials and Methods. The expression of β3-integrin subunits was detected by flow cytometry.
