Figure 1.

Daily alterations in ¹⁴C-paclitaxel within JIMT-1BR3 BMBC. (A) When examining all tumors independently, mice receiving paclitaxel injections at ZT14 or ZT17 demonstrated significantly increased ¹⁴C-paclitaxel concentrations within BMBC relative to mice receiving paclitaxel injections at ZT0 or ZT5. (B) When examining animal averages, planned comparisons demonstrate an increase in ¹⁴C-paclitaxel concentrations within BMBC at ZT17 relative to ZT0 or ZT 5. (C) Additionally, when combining groups and assessing animal averages based on activity, mice that received paclitaxel injections during their active phase displayed significantly increased ¹⁴C-paclitaxel concentrations within BMBC. (D) Representative cresyl violet and autoradiography images for ZT0 and ZT17. [(A) n= 122, 269, 185, 142 respectively; (B) n= 9, 9, 9, 8 respectively; (C) n= 18, 17 respectively]. The data are presented as mean +SEM. Graph bars that do not share a letter are statistically significantly different at p<0.05. ^#Main effect of time of injection; (A) nonparametric Kruskal-Wallis test; Dunn’s multiple comparisons test (B) one-way ANOVA; Fisher’s LSD multiple comparisons test (C) unpaired t-test.