Skip to main content
. 2021 Dec 9;11:752331. doi: 10.3389/fonc.2021.752331

Figure 2.

Figure 2

Optimal timing of chemotherapy delays onset of neurological symptoms and increases cell death within JIMT-1BR3 BMBC. (A) Mice harboring BMBC and receiving paclitaxel injections at ZT17 (ie TC-ZT17, n=15) demonstrated prolonged onset of neurological symptoms relative to mice harboring BMBC and receiving paclitaxel injections at ZT0 (i.e., TC-ZT0, n=16). Mice receiving paclitaxel injections alone did not display neurological deficits irrespective of administration time (i.e., C-ZT0, n=14 and C-ZT17, n=15). Additionally, mice harboring BMBC and receiving paclitaxel injections at ZT17 demonstrated significantly prolonged (B) mean and (C) median number of days until the onset of neurological symptoms and significantly increased (D) cell death with BMBC relative to ZT0. (E, F) Representative images of cell death within BMBC at ZT0 and ZT17. For negative controls see Supplemental Figures 1 and 2 . Green- cytokeratin 5/8/GFP, Red- cleaved caspase-3. (B. n= 16,15 respectively; (C) n= 16,15 respectively; (D) n= 11, 11 respectively). The data are presented as mean +SEM for (B, D) and median +95% CI for (C). Graph bars that do not share a letter are statistically significantly different at p<0.05. * Log-rank p<0.05. (A) Log-rank test (B) unpaired t-test (C) Mann Whitney test (D) unpaired t-test.