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. 2021 Aug 30;156(6):609–616. doi: 10.1007/s00418-021-02026-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Changes in cell numbers and junctional length during differentiation. a The changes in the number of cells during the differentiation of clone 1 are shown. The number of cells/0.1 mm² steadily increased until day 14, where a maximum of 211 ± 3 per 0.1 mm² was reached (n = 8, *p < 0.05, one-way ANOVA). bLikewise, the average number of cells per 0.1 mm² of clone 2 rose through the different states of differentiation (n = 8, *p < 0.05, one-way ANOVA). c. The increase in cell number was paralleled by an increase of the junctional length/µm. The junctional length of clone 1 increased from 0.10 ± 0.03 µm/0.1 mm² to 0.22 ± 0.04 µm/0.1 mm² at the state of full barrier (n = 8, *p < 0.05, one-way ANOVA). d. Similarly, in clone 2 the junctional length expanded over time (n = 8, *p < 0.05, one-way ANOVA)