Table 1.
Details of animals, isolates and strains.
| Animal | Geographic origin | Isolate | Pathology | MLST | spa | Strain ID according to array | PVL by lateral flow |
|---|---|---|---|---|---|---|---|
| A | Berlin | WT19 | Multifocal moderate to severe suppurative necrotising pneumonia and suppurative pyelonephritis. Found dead. | ST4614a | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?)b | Positive |
| B | Berlin | WT63 | Severe suppurative necrotising pneumonia and multiple small abscesses in spleen, kidney, caecum, mesenteric lymph nodes and intercostal muscles. Found dead. | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive |
| WT64 | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive | |||
| C (“Tiffy”) | Bavaria | WT65 | Severe abscessing mandibular lymphadenitis, severe suppurative cystitis. Died in a wildlife rehabilitation centre. | ST49 | t208 | CC49-MSSA (lukF-PV + /lukS-PV?) | Positive |
| D | Berlin | WT66 | Severe fibrinous-purulent myocarditis, suppurative pyelonephritis (right kidney only) and prostatitis. Found dead. | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive |
| WT67a | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive | |||
| WT67b | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive | |||
| WT68 | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive | |||
| WT69 | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive | |||
| E | Berlin | WT70 | Suppurative necrotising pneumonia, suppurative pyelonephritis and lymphadenitis (popliteal lymph nodes), multiple abscesses in caecum wall. Found dead. | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive |
| F | Berlin | WT71 | Abscesses subcutaneous (chest wall/axilla) and in popliteal lymph nodes. Euthanised due to poor physical condition. | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) | Positive |
| G | Berlin | WT110 | Suppurative necrotising pneumonia, enlarged thyroid gland containing small yellowish abscesses, suppurative pyelonephritis, splenomegaly, multiple abscesses in caecum and colon walls as well as in skin. Cachexia. Found dead. | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) c | Positive |
| WT111 | ST4614 | t3058 | CC1956-MSSA (lukF-PV + /lukS-PV?) c | Negative | |||
| H | Marchfeld Channel, Austria | B1 | Nasal swab. Good physical condition except for gunshot traumata including parts of a bullet in the right hindleg with surrounding acute necrotizing inflammation. All other organs unremarkable. | N/A | t085 | CC398-MSSA | N/A |
| I | Lower Austria | B2 | Abscessing lymph node, knee. Shot dead. | N/A | t008 | CC8-MSSA | N/A |
| J | Eisenstadt region, Austria | B3 | Pneumonia (Streptococcus pyogenes, Morganella morganii). Splenomegaly. S. aureus originated from enrichment culture of lung tissue. Found dead. | N/A | t394 | CC8-MSSA (sed/j/r +) | N/A |
| K | Lower Austria | B4 | Nasal Swab. Healthy animal shot dead. | N/A | t156 | CC12-MSSA (sep +) | N/A |
aST4614 is a single locus variant of ST1956; arcC-6, aroE-291, glpF-6, gmk-2, pta-7, tpi-225, yqiL-585.
bClearly positive for lukF-PV, weekly positive or ambiguous signal for lukS-PV.
cWT110 and WT111 differed in haemolysis on Columbia blood agar and were thus handled separately although array analysis eventually revealed identical strain affiliations.