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. 2021 Dec 9;12:793876. doi: 10.3389/fpls.2021.793876

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Copyright © 2021 Ponert, Šoch, Vosolsobě, Čiháková and Lipavská.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical detection of trehalase activity in D. majalis tissues. Blue colored formazan precipitate marks trehalase activity. Control incubations without added substrate did not stain (C,F,I). (A,C) Longitudinal sections of trehalose-supported asymbiotic protocorms, (B) glucose-supported asymbiotic protocorm, and (D-F) symbiotic protocorms cultured with Ceratobasidium sp. (G-I) cross sections of mycorrhizal roots of mature plants. The trehalase activity is present in the vicinity of pelotons (D,E,G,H). Scale bars 1mm.