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Fig. 3. (a) Fluorescence titration spectra of BNQ (5 μM) in DMSO/HEPES buffer (10 mM, pH = 6.0, 1/9, v/v) with the addition of Cu2+; (b) The fluorescence intensity ratio (F/F0) of BNQ is almost linearly related to the concentration of Cu2+ in range of 0–7.5 μM.