Gradient elution procedures.
| Time (min) | A: acetonitrile (%) | B: 0.1% formic acid water (%) |
|---|---|---|
| 0–12 | 5.0 | 95.0 |
| 12–15 | 5.0–15.0 | 95.0–85.0 |
| 15–45 | 15.0–35.0 | 85.0–65.0 |
| 45–50 | 35.0–50.0 | 65.0–50.0 |
| 50–60 | 50.0 | 50.0 |
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