Figure 4.

3D architecture of E. faecalis OG1RF::p23cfp biofilms with or without B. subtilis natto supernatant. The CFP-expressing strain E. faecalis OG1RF::p23cfp was cultured anaerobically in M9B medium without or with B. subtilis natto supernatant (0, 10, or 50% v/v) on glass coverslips in tissue culture dishes. After 24 or 48h of cultivation, the biofilms that formed on the bottom of the culture dishes were washed, fixed, and then analyzed using CLSM. The approximate biofilm thicknesses (μm) for all groups were measured and are shown in the figure.