Table 3.
Validation of RNA-seq results by RT-qPCR.
| Locus tag | Symbol | Annotation | FC (treated/control) | |
|---|---|---|---|---|
| aRNA-seq | RT-qPCR | |||
| OG1RF_RS01345 | copY | CopY/TcrY family copper transport repressor | 3.73 | 0.32±0.05 |
| OG1RF_RS01350 | ctpA | copper-translocating P-type ATPase | 0.45 | 0.26±0.01 |
| OG1RF_RS01870 | murQ | N-acetylmuramic acid 6-phosphate etherase | 0.45 | 0.41±0.11 |
| OG1RF_RS03760 | murD | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase | 0.41 | 0.80±0.15 |
| OG1RF_RS05035 | walK | cell wall metabolism sensor histidine kinase WalK | 0.30 | 0.49±0.03 |
| OG1RF_RS05115 | NADH peroxidase | 2.67 | 1.03±0.19 | |
| OG1RF_RS06740 | SidA/IucD/PvdA family monooxygenase | 0.31 | 0.25±0.15 | |
| OG1RF_RS08680 | murN | aminoacyltransferase | 0.25 | 0.25±0.01 |
| OG1RF_RS09175 | clpB | ATP-dependent chaperone ClpB | 0.47 | 0.86±0.19 |
| OG1RF_RS09795 | uppS | isoprenyl transferase | 0.38 | 0.41±0.03 |
| OG1RF_RS12995 | guaB | IMP dehydrogenase | 0.30 | 0.58±0.09 |
| OG1RF_RS13100 | cell wall surface anchor protein | 2.25 | 1.00±0.29 | |
| OG1RF_RS11220 | bgsA | cell wall glycolipid biosynthesis glucosyltransferase BgsA | 0.34 | 0.32±0.09 |
a
Relative gene expression normalized to gyrB. The 2−ΔΔCT method was used for calculations.