FIGURE 6.

CsWRKY48 binds to the promoter of CsGSTU8 and activates its expression. (A) EMSAs using purified GST and GST-CsWRKY48 proteins incubated together with biotin-labeled probes of W-box core elements (TGAC sequences) presented in CsGSTU8 promoter. -: absence; +: presence. (B) Schematics of the reporter and effector constructs used in the dual-LUC assays. (C) LUC signal detected in tobacco leaves. (D) LUC/REN ratio, as measured by the dual-LUC assays. (E) Transient expression of 35S::CsWRKY48-GFP and 35S::GFP in tea plant leaves. (F) RT-PCR was used to identify the transformation of 35S::CsWRKY48-GFP and 35S::GFP by using specific primers. (G) Expression of CsGSTU8 in transformed tea plant leaves. The data are presented as the means ± SDs of three independent experiments. Significant differences were determined using Student’s t-test (*P < 0.05).