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. 2021 Dec 9;14:790466. doi: 10.3389/fnmol.2021.790466

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Copyright © 2021 Ruff, Peters, Matsumoto, Ihle, Morales, Gaitanos, Yonehara, del Toro and Klein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flrt3 neuronal distribution in the mouse retina. (A) Scheme of the Flrt3-CreERT2 mouse. (B) Immunostaining showing Flrt3+ cells in magenta (tomato), RGCs in green (RBPMS), and the nucleus in white (DAPI). The white arrows indicate FLRT3-RGCs and the magenta arrows FLRT3-amacrine cells. (C) Plot showing the fractions of Flrt3-amacrine (AC) and FLRT3-RGCs in the Flrt3-CreERT2 mouse. Two complete retinas were counted and another three representative regions of interest (ROIs) of another two retinas were included in the quantification. (D) Retina sections of Flrt3-CreERT2;Tom^± mouse showing Flrt3 cells in purple and ChAT bands in green. The left panel shows the anatomical structure and cell types of a typical retinal section. The right panel shows a zoom of the IPL region and normalized intensity of ChAT and Flrt3 signals. AC, amacrine cells; BC, bipolar cells; HC, horizontal cells; RGC, retinal ganglion cell; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. (E) Representative retina density profile (%) of Flrt3-CreERT2;Tom^± mice. The left panel shows the distribution of Flrt3+ cells in the ganglion cell layer. The right panel shows the density of Flrt3+ RGCs identified from the left panel. N = 2 retinas from 2 mice (F) Random distribution of Flrt3-RGCs. Left panel: Flrt3-RGC distribution in a ROI of a retina region. Each dot represents a Flrt3+ RGC. Right panel: Density recovery profile of Flrt3+ RGC showing a random distribution. Graph shows Mean value ± SEM. Line represents a 7th order fit of the mean values. Shaded area is the 99% confidence interval. N = 4 retinas from 2 mice.